Inducible expression of a gene encoding an L41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast Candida maltosa.

AUTOR(ES)

Mutoh, E

RESUMO

In a previous paper (S. Kawai, S. Murao, M. Mochizuki, I. Shibuya, K. Yano, and M. Takagi, J. Bacteriol. 174:254-262, 1992), we showed that in each genome of several yeast species, there is one of two types of L41 gene, one for an L41 (Q-type) protein which confers cycloheximide (CYH) resistance or one for an L41 (P-type) protein which does not. These genes have been suggested to be responsible for the CYH response used in taxonomy. For example, Saccharomyces cerevisiae, which is CYH sensitive, has a P-type L41 gene, while Kluyveromyces fragilis and Candida maltosa, which are CYH resistant, have Q-type L41 genes. However, in contrast to K. fragilis, which is constitutively resistant to CYH, C. maltosa is inducibly resistant to CYH. Here, we show that C. maltosa has both types of the L41 gene in its genome and that expression of the Q-type L41 gene is induced by CYH while the P-type L41 gene is constitutively expressed.

Documentos Relacionados

• Yeast Cycloheximide-resistant crl Mutants Are Proteasome Mutants Defective in Protein Degradation
• Cycloheximide-Resistant Glycosylation in L Cells Infected with Chlamydia psittaci
• Cycloheximide-Resistant Temperature-Sensitive Lethal Mutations of Saccharomyces Cerevisiae
• Isolation and characterization of a cycloheximide-resistant mutant of Acanthamoeba castellanii Neff.
• Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts.