Inducible overproduction of the mouse c-myc protein in mammalian cells.
AUTOR(ES)
Wurm, F M
RESUMO
We have made Chinese hamster ovary (CHO) cell lines that contain up to 2000 copies of the coding region of the mouse c-myc gene fused to the promoter of the Drosophila gene (hsp70) encoding a Mr 70,000 heat shock protein. Incubation of these cells at 43 degrees C results in an estimated 100-fold induction of c-myc mRNA. Translation of this mRNA occurs when the cells are returned to 37 degrees C, and during the first 3 hr of recovery at 37 degrees C, the c-myc protein is one of the most abundantly synthesized proteins in the cells. The products of the induced c-myc gene are phosphoproteins of apparent Mr 64,000, 66,000, and 75,000. Induced cells die, suggesting that elevated levels of c-myc are cytotoxic. Amplification of genes placed under control of the Drosophila hsp70 promoter may provide a general method for inducibly over expressing proteins in mammalian cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=386297Documentos Relacionados
- c-Myc Proteolysis by the Ubiquitin-Proteasome Pathway: Stabilization of c-Myc in Burkitt's Lymphoma Cells
- Immortalization of mouse neural precursor cells by the c-myc oncogene.
- Immortalization of mouse neural precursor cells by the c-myc oncogene
- Metabolism of c-myc gene products: c-myc mRNA and protein expression in the cell cycle.
- Regulation of translocated c-myc genes transfected into plasmacytoma cells.