INDUCTION OF LYSOGENIC MICROCOCCUS LYSODEIKTICUS1

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Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129–1133. 1962.—Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold increase in infective-center concentration. Cells in the stationary growth phase and cells adapted to a chemically defined medium were also induced by ultraviolet irradiation. Dimethyl sulfate induced the lysogenic culture to about the same extent as did ultraviolet light, whereas β-propiolactone was less effective.

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