Infectivity of Chlamydia trachomatis in tissue culture with newborn calf serum.
AUTOR(ES)
LaScolea, L J
RESUMO
By using representative strains of Chlamydia trachomatis serotypes, the efficacy of substituting fetal bovine serum with newborn calf serum for the isolation of this organism in tissue culture was examined. The criteria used were the quality and quantity of the iodine-stained cytoplasmic inclusions and the characteristics of the McCoy cells. Complete substitution of fetal bovine serum with newborn calf serum produced a detrimental change in the quality of the cytoplasmic inclusions and a dramatic decline in the inclusion count (P less than 0.001) with all of the chlamydial strains tested. There appeared to be no significant alteration in the characteristics of the McCoy cells. It is recommended from this preliminary investigation that newborn calf serum should not be used for the isolation of C. trachomatis in tissue culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272220Documentos Relacionados
- A study of inactivation of Chlamydia trachomatis by normal human serum.
- Susceptibility of different serovars of Chlamydia trachomatis to inactivation by normal human serum.
- Staining of surface antigens of Chlamydia trachomatis L2 in tissue culture.
- Suppression of Chlamydia trachomatis inclusion formation by fetal calf serum in cycloheximide-treated McCoy cells.
- Antibody to Viruses Affecting Cattle in Commercial Tissue Culture Grade Fetal Calf Serum1
