Influence of Cold Acclimation on Membrane Injury in Frozen Plant Tissue
AUTOR(ES)
Stout, Darryl G.
RESUMO
Cold-acclimated twigs of Amelanchier alnifolia Nutt. released less HCN at −4.5 C than nonacclimated twigs following slow freezing to −25 C or rapid freezing to −78 C. Cold-acclimated twigs frozen slowly to −25 C released more HCN than cold-acclimated twigs frozen only to −4.5 C. Cold-acclimated twigs frozen slowly to −25 C and then rapidly to −78 C released less HCN at −4.5 C than cold-acclimated twigs frozen rapidly to −78 C. In general, K+ efflux and the inability to reduce triphenyl tetrazolium chloride following freezing and thawing paralleled HCN release at −4.5 C. Because low K+ efflux and high triphenyl tetrazolium chloride reduction are known to depend upon membrane integrity, the increased K+ efflux and the decreased triphenyl tetrazolium chloride reduction following freezing and thawing provide indirect evidence that HCN release at −4.5 C is a measure of membrane damage in frozen cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=425923Documentos Relacionados
- Cold Acclimation of Arabidopsis thaliana (Effect on Plasma Membrane Lipid Composition and Freeze-Induced Lesions).
- Effect of Cold Acclimation on Bulk Tissue Electrical Impedance: I. Measurements with Birdsfoot Trefoil at Subfreezing Temperatures
- Phospholipid Degradation in Frozen Plant Cells Associated with Freezing Injury 12
- Relationship of Electrical Conductance at Two Frequencies to Cold Injury and Acclimation in Cornus stolonifera Michx. 1
- Plasma Membrane Alterations in Callus Tissues of Tuber-bearing Solanum Species during Cold Acclimation 1
