Influence of osmolarity of the growth medium on the outer membrane protein pattern of Escherichia coli.
AUTOR(ES)
Alphen, W V
RESUMO
Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, respectively. Kinetic studies showed that, after the osmolarity of the medium was changed, one protein was hardly incorporated into the membrane, whereas the other was incorporated with an increased rate. After about 1.5 to 2 generations, the cell envelopes obtained the b/c ratio characteristic for the new medium, and both proteins were subsequently incorporated in the cell ensured this new ratio. Once proteins b and c were incorporated in the cell envelope, they were not converted into each other by changes in osmolarity of the growth medium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235471Documentos Relacionados
- Effect of growth medium on the relative polypeptide composition of cellular outer membrane and released outer membrane material in Escherichia coli.
- Alterations in Protein Expression Caused by the hha Mutation in Escherichia coli: Influence of Growth Medium Osmolarity
- Regulation of the OmpA outer membrane protein of Escherichia coli.
- Role of a major outer membrane protein in Escherichia coli.
- Periplasmic maltose-binding protein confers specificity on the outer membrane maltose pore of Escherichia coli.
