Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.

AUTOR(ES)

Li, S Q

RESUMO

Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.

Documentos Relacionados

• Hemolysis by Liposomes Containing Influenza Virus Hemagglutinins
• Generation of Influenza A Viruses with Chimeric (Type A/B) Hemagglutinins
• Vaccination with inactivated influenza A virus during pregnancy protects neonatal mice against lethal challenge by influenza A viruses representing three subtypes.
• Influenza C virus hemagglutinin: comparison with influenza A and B virus hemagglutinins.
• High-efficiency formation of influenza virus transfectants.