INHIBITION OF A THYMINE-DEFICIENT MUTANT OF ESCHERICHIA COLI BY 5-SUBSTITUTED URACILS

AUTOR(ES)

Shapira, Jacob

RESUMO

Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919–923. 1962.—Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli were incubated in a thymine-containing glucose-salts medium with a variety of 5-substituted pyrimidines and pyrimidine ribosides. After a lag phase, the turbidity of the cultures increased appreciably which, in the case of 5-ethyluracil and 5-ethyluridine, was primarily due to an elongation of the cells. 5-Ethyluracil at low thymine concentrations increased the lag phase and decreased the rate and final amount of growth. At high thymine concentrations, it had less effect on the final turbidity of the cultures. The inhibition index for this compound was relatively constant, suggesting competitive inhibition.

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