Inhibition of Escherichia coli viability by external guide sequences complementary to two essential genes

AUTOR(ES)

McKinney, Jeffrey

FONTE

The National Academy of Sciences

RESUMO

Narrow spectrum antimicrobial activity has been designed to reduce the expression of two essential genes, one coding for the protein subunit of RNase P (C5 protein) and one for gyrase (gyrase A). In both cases, external guide sequences (EGS) have been designed to complex with either mRNA. Using the EGS technology, the level of microbial viability is reduced to less than 10% of the wild-type strain. The EGSs are additive when used together and depend on the number of nucleotides paired when attacking gyrase A mRNA. In the case of gyrase A, three nucleotides unpaired out of a 15-mer EGS still favor complete inhibition by the EGS but five unpaired nucleotides do not.

Documentos Relacionados

• Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P
• Cross-clade inhibition of HIV-1 replication and cytopathology by using RNase P-associated external guide sequences
• RNase P-mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences
• tRNA nucleotidyltransferase is not essential for Escherichia coli viability.
• Disruption of type III secretion in Salmonella enterica serovar Typhimurium by external guide sequences