Inhibition of influenza virus production in virus-infected mice by RNA interference
AUTOR(ES)
Ge, Qing
FONTE
National Academy of Sciences
RESUMO
Influenza A virus infection is a major source of morbidity and mortality worldwide. Because the effectiveness of existing vaccines and antiviral drugs is limited, development of new treatment modalities is needed. Here, we show that short interfering RNAs (siRNAs) specific for conserved regions of influenza virus genes can prevent and treat influenza virus infection in mice. Virus production in lungs of infected mice is reduced by siRNAs given either before or after initiating virus infection, by using slow i.v. administration of small volumes containing siRNAs in complexes with a polycation carrier. Similar effects also are observed when mice are given DNA vectors i.v. or intranasally, from which siRNA precursors can be transcribed. Development of delivery systems that may be compatible with human use demonstrates the potential utility of siRNAs for prophylaxis and therapy of influenza virus infections in humans.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=423254Documentos Relacionados
- Inhibition of Virus Production in JC Virus-Infected Cells by Postinfection RNA Interference
- Production of Macrophage Migration Inhibition Factors by Virus-Infected Cell Cultures
- Interleukin-4 causes delayed virus clearance in influenza virus-infected mice.
- Characterization of polysome-associated RNA from influenza virus-infected cells.
- Appearance of Rosette-Forming Macrophages in the Lungs of Influenza Virus-Infected Mice