Inhibition of PCR by Agar from Bacteriological Transport Media

AUTOR(ES)

Gibb, Alan Patrick

FONTE

American Society for Microbiology

RESUMO

We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

Documentos Relacionados

• GELRITE as an Agar Substitute in Bacteriological Media
• Value of the K+ salt of carageenan as an agar substitute in routine bacteriological media.
• Contamination of Bacteriological Media by Leptospira biflexa
• Inhibition of Histoplasma capsulatum by Candida albicans and Other Yeasts on Sabouraud's Agar Media
• Recovery of Agar from Used Media