Inhibition of rat corneal angiogenesis by a nuclease-resistant RNA aptamer specific for angiopoietin-2
AUTOR(ES)
White, Rebekah R.
FONTE
The National Academy of Sciences
RESUMO
Angiopoietin-2 (Ang2) appears to be a naturally occurring antagonist of the endothelial receptor tyrosine kinase Tie2, an important regulator of vascular stability. Destabilization of the endothelium by Ang2 is believed to potentiate the actions of proangiogenic growth factors. To investigate the specific role of Ang2 in the adult vasculature, we generated a nuclease-resistant RNA aptamer that binds and inhibits Ang2 but not the related Tie2 agonist, angiopoietin-1. Local delivery of this aptamer but not a partially scrambled mutant aptamer inhibited basic fibroblast growth factor-mediated neovascularization in the rat corneal micropocket angiogenesis assay. These in vivo data directly demonstrate that a specific inhibitor of Ang2 can act as an antiangiogenic agent.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=154292Documentos Relacionados
- Nuclease-resistant synthetic ribozymes: developing a new class of therapeutics
- Bioactive and nuclease-resistant l-DNA ligand of vasopressin
- Nuclease-resistant chimeric ribozymes containing deoxyribonucleotides and phosphorothioate linkages.
- Extensive phosphorothioate substitution yields highly active and nuclease-resistant hairpin ribozymes.
- Nuclease-resistant ribozymes decrease stromelysin mRNA levels in rabbit synovium following exogenous delivery to the knee joint.
