Inhibition of SV40 gene expression by microinjected small antisense RNA and DNA molecules
AUTOR(ES)
Graessmann, M.
RESUMO
We tested the impact of antisense RNA and DNA molecules on SV40 gene expression by microinjection into TC7 cells. Short antisense stretches, complementary to either hairpin or loop structures on the T antigen mRNA, inhibited T antigen synthesis. In contrast, full-length antisense RNA and DNA molecules did not effect T antigen synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=333533Documentos Relacionados
- Inhibition of SV40 replicon function by engineered antisense RNA transcribed by RNA polymerase III.
- Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
- Inhibition of SV40 T antigen formation by interferon.
- Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
- Transcription of nucleosomal DNA in SV40 minichromosomes by eukaryotic and prokaryotic RNA polymerases.