Initial Steps in the Degradation of Phosphinothricin (Glufosinate) by Soil Bacteria
AUTOR(ES)
Bartsch, Klaus
RESUMO
Three hundred bacterial isolates from soil were tested for resistance against phosphinothricin [PPT; dl-homoalanin-4-yl(methyl)phosphinic acid], the active ingredient of the herbicide BASTA. Eight resistant bacterial strains and Escherichia coli were analyzed for PPT-transforming activities. At least three different enzymatic reactions could be detected in cell extracts. In six strains an acetyltransferase was active, synthesizing N-acetyl-PPT in the presence of PPT and acetyl coenzyme A. All strains could degrade PPT to its corresponding 2-oxoacid {2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid} by transamination. Rhodococcus sp., the only tested strain that was able to utilize PPT as a sole source of nitrogen, formed 2-oxo-4[(hydroxy)(methyl)phosphinoyl]butyric acid by oxidative deamination. This enzymatic activity was inducible by l-glutamic acid or PPT itself but not in the presence of NH4+. d-PPT transformation was not detectable in any of the investigated strains.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=184185Documentos Relacionados
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