Initiation and termination of deoxyribonucleic acid replication in bacteria after a stepwise increase in the velocity of replication.
AUTOR(ES)
Bremer, H
RESUMO
The theoretical relations between replication, initiation, termination, and deoxyribonucleic acid (DNA) accumulation were derived for experiments in which the length of the time required for the replication of the bacterial chromosome (C period) can be varied. This theory enables one to determine absolute values of the C period from kinetics of DNA accumulation after a "stepup" with thymine-requiring bacteria that are subjected to a sudden increase in the exogenous thymine concentration. Application of this method of data evaluation to an observed step-up experiment with a thy-derivative of Escherichia coli B/r (ATCC 12407) indicated that the theory describes the observed post-step accumulation of DNA accurately within experimental errors. It is also concluded that changes in the replication velocity (C) do not measurably affect the timing of initiation events in a culture.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=235177Documentos Relacionados
- Initiation and Termination of Bacterial Deoxyribonucleic Acid Replication in Low Concentrations of Chloramphenicol
- The spatial organization of sequences involved in initiation and termination of eukaryotic DNA replication.
- Initiation and termination of chromosome replication at 45 degree C in a temperature-sensitive deoxyribonucleic acid initiation mutant of Bacillus subtilis 168, TsB134.
- Deoxyribonucleic acid-membrane interactions near the origin of replication and initiation of deoxyribonucleic acid synthesis in Escherichia coli.
- Deoxyribonucleic acid synthesis after inhibition of initiation of rounds of replication in Escherichia coli B/r.
