Initiation Characteristics for the Synthesis of Five T4 Phage-Specific Messenger RNAs In Vitro
AUTOR(ES)
Natale, Peter J.
RESUMO
The involvement of the nucleoside triphosphates in the initiation of the synthesis of the messenger ribonucleic acid of five T4 specific enzymes has been studied. Only one of these, the messenger RNA for deoxynucleosidemonophosphate kinase, can be initiated in the presence of one nucleoside triphosphate, namely ATP. All of the remaining four require the presence of at least two nucleoside triphosphates during the initiation period. The combination of ATP and UTP was best for the initiation of messenger RNA for dihydrofolate reductase, ATP and CTP for deoxycytidylate hydroxymethyltransferase and β-glucosyltransferase, and ATP and GTP for α-glucosyltransferase. We have concluded that there is a great variation in the nucleotide composition and sequence of the initiation sites in T4 DNA. No correlation in the requirements of nucleoside triphosphates during the initiation period could be observed among the five systems studied according to their classification as one type or another of “early” T4 messenger RNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388018Documentos Relacionados
- DNA-Directed Synthesis In Vitro of T4 Phage-Specific Enzymes
- SYNTHESIS OF PHAGE-SPECIFIC α- AND β-GLUCOSYL TRANSFERASES DIRECTED BY T-EVEN DNA in vitro*
- The role of the N gene of phage lambda in the synthesis of two phage-specific proteins.
- In vitro synthesis of T4 late messenger RNA.
- Enzyme associations in T4 phage DNA precursor synthesis