Initiation Factor 2 of Myxococcus xanthus, a Large Version of Prokaryotic Translation Initiation Factor 2
AUTOR(ES)
Tiennault-Desbordes, Emmanuelle
FONTE
American Society for Microbiology
RESUMO
We have isolated the structural gene for translation initiation factor IF2 (infB) from the myxobacterium Myxococcus xanthus. The gene (3.22 kb) encodes a 1,070-residue protein showing extensive homology within its G domain and C terminus to the equivalent regions of IF2 from Escherichia coli. The protein cross-reacts with antibodies raised against E. coli IF2 and was able to complement an E. coli infB mutant. The M. xanthus protein is the largest IF2 known to date. This is essentially due to a longer N-terminal region made up of two characteristic domains. The first comprises a 188-amino-acid sequence consisting essentially of alanine, proline, valine, and glutamic acid residues, similar to the APE domain observed in Stigmatella aurantiaca IF2. The second is unique to M. xanthus IF2, is located between the APE sequence and the GTP binding domain, and consists exclusively of glycine, proline, and arginine residues.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=94867Documentos Relacionados
- Nutrition of Myxococcus xanthus, a fruiting myxobacterium.
- Tyrosine phosphorylation in Myxococcus xanthus, a multicellular prokaryote.
- The dsg gene of Myxococcus xanthus encodes a protein similar to translation initiation factor IF3.
- The Myxococcus xanthus dsg gene product performs functions of translation initiation factor IF3 in vivo.
- The Morphology and Cytology of Myxococcus xanthus, N. Sp. 1
