INORGANIC POLYPHOSPHATE METABOLISM IN CHLOROBIUM THIOSULFATOPHILUM
AUTOR(ES)
Hughes, D. E.
RESUMO
Hughes, D. E. (Dartmouth Medical School, Hanover, N.H.), S. F. Conti, and R. C. Fuller. Inorganic polyphosphate metabolism in Chlorobium thiosulfatophilum. J. Bacteriol. 85:577–584. 1963.—Cells of the obligate phototroph Chlorobium thiosulfatophilum, when grown on a normal concentration of inorganic phosphate, accumulated large intracellular metachromatic granules identified as polymetaphosphate. Inorganic phosphate was released from polymetaphosphate by cell-free extracts. This release was adenosine diphosphate-dependent and light-independent. When cells were subcultured through serial transfer in the absence of inorganic phosphate, the production of polymetaphosphate granules was almost completely stopped. After the serial transfer, these cells showed little or no endogenous release of inorganic phosphate or release from added polymetaphosphate. Using such polymetaphosphate-free cells, it has been possible to demonstrate photosynthetic phosphorylation by the naturally occurring photosynthetic macro-molecules isolated from this organism. These particles, of about 100 A in diameter with a molecular weight of approximately 1.5 million, are by far the simplest functional naturally occurring photosynthetic electron-transport units thus far described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=278185Documentos Relacionados
- Control of Oxidative Sulfur Metabolism of Chlorobium limicola forma thiosulfatophilum
- Structure of cytochrome c555 of Chlorobium thiosulfatophilum: primitive low-potential cytochrome c.
- Degradation of Inorganic Polyphosphate in Mutants of Aerobacter aerogenes
- Inorganic polyphosphate in the origin and survival of species
- Inorganic polyphosphate kinase and adenylate kinase participate in the polyphosphate:AMP phosphotransferase activity of Escherichia coli