Insertion of pr5k gene in sugar cane to induce resistant plants to rust disease fungi puccinia melanocephala. / Inserção do gene PR5K em cana-de-açúcar visando induzir resistência ao fungo da ferrugem Puccinia melanocephala.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

The Sugar cane is very important culture for many countries especially Brazil. This culture however presents many problems with diseases; mainly rust disease that is spread mechanically and caused by Puccinia melanocephala fungi. The aim of this work was to get resistant plants with the gene PR5K , which is related to PR5 resistant proteins. Embriogenic callus of sugar cane plants Australian variety Q117 were used in shot gun procedure using tungsten particles covered by DNA from pRGA vector. The construction were made using the plasmid pAHC17 that contains the maize ubiquitin gene that is expressed only in monocotyledons plants, the plasmid pXL3 with PR5K gene related with PR5 proteins, involved with the defense system in plants. The new plasmid has part of pHA9 that contains the neo gene, which allows the plant to be resistant to geneticin used in the selection procedure. The plasmid pRGA was used for the short gum experiments. Callus with 19 weeks were transferred to manitol and sorbitol media, 4 hours before shooting. After shooting the calluses were transferred to CI-3-2,4-D media plus geneticin antibiotic (35 mg/mL). The selected plants were sub cultivated in the CI-3BAP media. From the total callus used in the shot gun experiments, 20% were selected, and from this total, 1% gave positive result using the PCR procedure, indicating that the transformed plants has the pRGA plasmid.

ASSUNTO(S)

transgenic plants biolística fungos fitopagênicos genes cana-de-açúcar pathogenic fungi plantas transgênicas ferrugem (doença de planta) resistência genética vegetal. rust (plant disease) sugar cane biolistic genes plant genetic resistance.

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