Insertion site preferences of the P transposable element in Drosophila melanogaster
AUTOR(ES)
Liao, Guo-chun
FONTE
The National Academy of Sciences
RESUMO
We determined the genomic sequence at the site of insertion in 2,266 unselected P element insertion events. Estimating physical properties of the genomic DNA at these insertion sites—such as base composition, bendability, A-philicity, protein-induced deformability, and B-DNA twist—revealed that they differ significantly from average chromosomal DNA. By examining potential hydrogen bonding sites in the major groove, we identified a 14-bp palindromic pattern centered on the 8-bp target site duplication that is generated by P element insertion. Our results suggest that the P-element transposition mechanism has a two-fold dyad symmetry and recognizes a structural feature at insertion sites, rather than a specific sequence motif.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=16242Documentos Relacionados
- Effects of a Transposable Element Insertion on Alcohol Dehydrogenase Expression in Drosophila Melanogaster
- P-Element-Induced Recombination in Drosophila Melanogaster: Hybrid Element Insertion
- A Recent Adaptive Transposable Element Insertion Near Highly Conserved Developmental Loci in Drosophila melanogaster
- Insertion and Excision of the Transposable Element Mariner in Drosophila
- Capture of flanking DNA by a P element in Drosophila melanogaster: creation of a transposable element.