Integrator3, a Partner of Single-stranded DNA-binding Protein 1, Participates in the DNA Damage Response*
AUTOR(ES)
Zhang, Feng
FONTE
American Society for Biochemistry and Molecular Biology
RESUMO
Single-stranded DNA-binding protein 1 (SSB1) plays an important role in the DNA damage response and maintenance of genomic stability. Here, by using protein affinity purification, we have identified Integrator3 (INT3) as a novel partner of SSB1. INT3 forms a complex with SSB1 by constitutively interacting with SSB1 regardless of DNA damage. However, following DNA damage, along with SSB1, INT3 relocates to the DNA damage sites and regulates the accumulation of TopBP1 and BRCA1 there. Moreover, INT3 controls DNA damage-induced Chk1 activation and G2/M checkpoint activation. In addition, INT3 is involved in homologous recombination repair by regulating Rad51 foci formation following DNA damage. Taken together, these results demonstrate that INT3 plays a key role in the DNA damage response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2781595Documentos Relacionados
- The single-stranded DNA-binding protein of Escherichia coli.
- The single-stranded DNA-binding protein of Deinococcus radiodurans
- Minisatellite binding protein Msbp-1 is a sequence-specific single-stranded DNA-binding protein.
- Major coat protein and single-stranded DNA-binding protein of filamentous virus Pf3.
- Identification and Characterization of the Single-Stranded DNA-Binding Protein of Bacteriophage P1