Interaction of the fish pathogen Aeromonas salmonicida with rainbow trout macrophages.
AUTOR(ES)
Garduño, R A
RESUMO
A procedure was developed to culture rainbow trout macrophages (M phi) on supported glass coverslips. Using this method and a variety of well-characterized Aeromonas salmonicida strains with normal or altered cell surfaces, we investigated the role of this unusual bacterial surface in the bacterium-M phi interaction. An intact crystalline protein array, the A-layer, mediated adherence of A. salmonicida cells to M phi even in the absence of opsonins. In contrast, unopsonized cells of an A-layer-negative (A-) mutant with a smooth lipopolysaccharide (LPS) layer were unable to interact with M phi. However, this ability was recovered when the A-layer was reconstituted onto the smooth LPS surface of these A- LPS+ cells. Two A. salmonicida mutants possessing the A-layer in different disorganized states had a reduced ability to interact with M phi. A+ cells grown under calcium limitation produced A-layers locked into an alternative conformation which mediated the highest levels of M phi association in the absence of opsonins or any other surface coating. Coating A+ cells with hemin greatly increased their levels of M phi association, and bacterial cells grown on trout blood agar plates also had a dramatic increase in their ability to interact with M phi. Only A+ A. salmonicida cells were highly cytotoxic to trout M phi, especially after being coated with hemin, presumably due to a more focused targeting of the bacterial cell onto the M phi surface and/or into the intracellular regions of the M phi.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=258210Documentos Relacionados
- S-layer-mediated association of Aeromonas salmonicida with murine macrophages.
- A conserved Aeromonas salmonicida porin provides protective immunity to rainbow trout.
- In vitro susceptibility of the fish pathogen Aeromonas salmonicida to flumequine.
- In vitro activities of 4-quinolones against the fish pathogen Aeromonas salmonicida.
- gyrA mutations in quinolone-resistant isolates of the fish pathogen Aeromonas salmonicida.