Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.
AUTOR(ES)
King, D S
RESUMO
PRP2 protein of Saccharomyces cerevisiae is required for the pre-mRNA splicing reaction but not for the early stages of spliceosome assembly. Using anti-PRP2 antibodies we demonstrate that PRP2 protein is associated with spliceosomes prior to, and throughout step 1 of the splicing reaction. Heat-inactivated prp2 protein, by contrast, does not seem to associate with spliceosomes. By elution of electrophoretically distinct spliceosomal complexes from non-denaturing gels we identify the specific complex with which PRP2 initially interacts in the pathway of spliceosome assembly.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=332610Documentos Relacionados
- The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.
- The purified yeast pre-mRNA splicing factor PRP2 is an RNA-dependent NTPase.
- Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae.
- Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing.
- The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors.