Intercellular contact-dependent survival of human A549, NCI-H596 and NCI-H520 non-small cell lung carcinoma cell lines
AUTOR(ES)
Castro, Mauro Antônio Alves
DATA DE PUBLICAÇÃO
2010
RESUMO
In the present study, we examined the relationship between cell phenotype and cell survival of three human non-small cell lung carcinoma cell lines (A549, NCI-H596 and NCI-H520). Cells in exponential growth at various densities were incubated for 24 h at 37ºC in a 5% CO2 humidified atmosphere and then exposed to UV radiation for 1 min (256 nm, 40 W, source-to-target distance 100 cm). After two days the surviving cells were quantified by sulforhodamine ß staining and DNA fragmentation assay. The differences in UV sensitivity at 60 x 103 cells/cm2 among the cell lines were not related to the proliferative state of the cells but to the extent of intercellular contact. In contrast to A549 and NCI-H596, irradiated NCI-H520 cells presented lower DNA fragmentation and an aggregated cell culture phenotype even prior to confluence, suggesting that a contact-effect mechanism provides further protection against UV radiation.
ASSUNTO(S)
neoplasias pulmonares non-small cell lung carcinoma carcinoma pulmonar de celula nao-pequena cell density intercellular contact contagem de células cell phenotype ciclo celular uv radiation fenótipo tolerância à radiação
ACESSO AO ARTIGO
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