Intergeneric Complementation of Anthranilate Synthase Subunits

AUTOR(ES)

Patel, Nanu

RESUMO

Partially purified subunits of anthranilate synthase were prepared from Bacillus subtilis and Pseudomonas aeruginosa. The large component from B. subtilis (IB) complements well with the small component from P. aeruginosa (IIP) to reconstitute a glutamine-reactive anthranilate synthase. This interaction can be demonstrated with crude extracts from a B. subtilis trpX mutant and a P. aeruginosa trpA mutant. Complementation was also observed with the large component from P. aeruginosa (IP) and the small subunit from B. subtilis (IIB). At saturation the heterologous complex IBIIP has 93% of the activity of the homologous complex IBIIB, whereas the hybrid IPIIB is only 22% as active as the homologous complex IPIIP.

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