Intracellular forms of adenovirus DNA. V. Viral DNA sequences in hamster cells abortively infected and transformed with human adenovirus type 12.
AUTOR(ES)
Fanning, E
RESUMO
The persistence of viral DNA in BHK-21 cells abortively infected with human adenovirus type 12 has been investigated using reassociation kinetics. No indication of an increase in the amount of viral DNA per cell has been found. On the contrary, the amount of intracellular viral DNA sequences decreases rapidly after infection. Thus, free adenovirus type 12 DNA does not replicate in BHK-21 cells. The influence of the multiplicity of infection on the amount of persisting adenovirus type 12 DNA has also been explored. The viral DNA sequences persisting in four lines of hamster cells transformed in vitro by adenovirus type 12 at various multiplicities of infection have been quantitated and mapped by reassociation kinetics experiments using restriction endonuclease fragments of 3H-labeled adenovirus type 12 DNA. All the EcoRI restriction nuclease fragments of the adenovirus type 12 genome are represented in each of the four cell lines. Individual fragments of the viral genome are represented in multiple copies in non-equimolar amounts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355005Documentos Relacionados
- Intracellular Forms of Adenovirus DNA V. Viral DNA Sequences in Hamster Cells Abortively Infected and Transformed with Human Adenovirus Type 12
- Transcription of the genome of adenovirus type 12. I. Viral mRNA in abortively infected and transformed cells.
- Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells.
- Degradation of intracellular DNA in KB cells infected with cyt mutants of human adenovirus type 12.
- Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12.