Intracellular Location of Carbonate Dehydratase (Carbonic Anhydrase) in Leaf Tissue

AUTOR(ES)

Reed, Malcolm Leonard

RESUMO

Two proteins which have carbonate dehydratase (carbonic anhydrase, EC 4.2.1.1) activity were shown to be in the chloroplasts and in the cytosol of leaves of Brassica chinensis, Spinacia oleracea, and in variegated leaves of Tradescantia albiflora and Hedera canariensis. The chloroplastic enzyme is smaller than the one in the cytosol, as it runs farther on gradient polyacrylamide gels. It was separated from the other by isolation of chloroplasts of Brassica and Spinacia on sucrose density gradients; approximately half of the total activity was in the chloroplasts.

Documentos Relacionados

• Identification of Intracellular Carbonic Anhydrase in Chlamydomonas reinhardtii with a Carbonic Anhydrase-Directed Photoaffinity Label.
• Intracellular carbonic anhydrase of Chlamydomonas reinhardtii.
• Carbonic anhydrase inhibitors and osmotic agents in glaucoma. Carbonic anhydrase inhibitors.
• Carbonic Anhydrase of Spinach: Studies on Its Location, Inhibition, and Physiological Function 1
• The mouse carbonic anhydrase I gene contains two tissue-specific promoters.