Intracellular location of the histonelike protein HU in Escherichia coli.
AUTOR(ES)
Dürrenberger, M
RESUMO
Immunocytochemical labeling of thin sections of cryosubstituted, Lowicryl-embedded Escherichia coli cells with protein A-colloidal gold was used to study the structural organization of the bacterial nucleoid. We found that the histonelike protein HU was not associated with the bulk DNA in the nucleoid but was located in areas of the cell where metabolically active DNA is associated with ribosomes and where single-stranded DNA, RNA polymerase, and DNA topoisomerase I were also located. The resolution of the methods used did not allow us to decide whether HU was associated either with ribosomes or with transcriptionally active DNA, nor could we demonstrate interaction of HU with either.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=211518Documentos Relacionados
- Intracellular location of catabolite activator protein of Escherichia coli.
- Transcriptional activation of bacteriophage lambda DNA replication in vitro: regulatory role of histone-like protein HU of Escherichia coli.
- Alterations of the outer membrane composition in Escherichia coli lacking the histone-like protein HU
- Lon-dependent regulation of the DNA binding protein HU in Escherichia coli.
- Protein HU in the enzymatic replication of the chromosomal origin of Escherichia coli.