Intracellular Viral RNA Species in Mouse Cells Nonproductively Transformed by the Murine Sarcoma Virus
AUTOR(ES)
Tsuchida, Nobuo
RESUMO
The size and quantity of virus-specific RNA in five non-virus-producing mouse cells transformed by the Moloney isolate of murine sarcoma virus (MSV) was determined. Hybridization of RNA from transformed cells with the [3H]DNA product of the RNA-directed DNA polymerase of the murine sarcoma-leukemia virus was used to detect and quantitate virus-specific RNA. The amount of virus-specific RNA in non-virus-producing cells was less than one-sixth of that found in virus-producing cells. A striking correlation was found between the amount of intracellular virus-specific RNA and the degree of agglutination by conconavalin A previously reported for the four non-virus-producing NIH/3T3 cell lines (Salzberg and Green, 1974). A major RNA subunit sedimenting at 26 to 28S was detected in all five MSV-transformed non-virus-producing cells. This could represent the RNA genome of defective MSV.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=355553Documentos Relacionados
- Absence of a Specific Ganglioside Galactosyltransferase in Mouse Cells Transformed by Murine Sarcoma Virus
- COMPLEMENTARY NUCLEAR RNA'S OF MURINE SARCOMA-LEUKEMIA VIRUS COMPLEX IN TRANSFORMED CELLS*
- Expression of the Major Internal Viral Polypeptide in Cells Transformed by Wild-Type and Temperature-Sensitive Murine Sarcoma Virus
- In vitro production of immunosuppressive factors by murine sarcoma virus-transformed mouse fibroblasts.
- Production of Virus by Mammalian Cells Transformed by Rous Sarcoma and Murine Sarcoma Viruses