Introns are inconsequential to efficient formation of cellular thymidine kinase mRNA in mouse L cells.
AUTOR(ES)
Gross, M K
RESUMO
TK mRNA levels were determined in mouse L cells transformed with intron deletion mutations of the chicken TK gene. Whether normalized per cell, per integrated gene, or per internal control signal, intron deletion did not diminish the efficiency of TK mRNA formation in transformed L cells. The results demonstrated that introns are not required for efficient biogenesis of cellular mRNA in transformed mouse L cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368147Documentos Relacionados
- Induction of cellular thymidine kinase occurs at the mRNA level.
- Cytoplasmic delivery of ribozymes leads to efficient reduction in alpha-lactalbumin mRNA levels in C127I mouse cells.
- Control of thymidine kinase mRNA during the cell cycle.
- mRNA stability in mammalian cells.
- Insulin is essential for accumulation of casein mRNA in mouse mammary epithelial cells.