Invasion of respiratory epithelial cells by Burkholderia (Pseudomonas) cepacia.
AUTOR(ES)
Burns, J L
RESUMO
Pulmonary infections caused by Burkholderia (Pseudomonas) cepacia are an important cause of morbidity and mortality in cystic fibrosis (CF) patients. Several features suggestive of cellular invasion and intracellular sequestration of B. cepacia in CF are persistence of infection in the face of antibiotic therapy to which the organism demonstrates in vitro susceptibility and a propensity to cause bacteremic infections in patients with CF. Epithelial cell invasion was demonstrated in vitro in A549 cells by a modified gentamicin protection assay. The kinetics of invasion appear to be saturable. Electron microscopy of invaded monolayers showed intracytoplasmic bacteria enclosed by membrane-bound vacuoles. No lysosomal fusion with these vacuoles was observed. Intraepithelial cell replication was suggested by electron microscopy and confirmed by both a quantitative assay and a visual assay. Cytochalasin D, but not colchicine, inhibited invasion, suggesting a role for microfilaments but not microtubules. The invasion phenotype in B. cepacia may be an important virulence factor for CF infections.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=174336Documentos Relacionados
- Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia.
- Structurally variant classes of pilus appendage fibers coexpressed from Burkholderia (Pseudomonas) cepacia.
- Induction of biologically active interleukin-8 from lung epithelial cells by Burkholderia (Pseudomonas) cepacia products.
- Genetic structure of a lotic population of Burkolderia (Pseudomonas) cepacia.
- Priming of neutrophil respiratory burst activity by lipopolysaccharide from Burkholderia cepacia.
