Inverted repeats of Tn5 are transposable elements.
AUTOR(ES)
Berg, D E
RESUMO
Experiments presented here show that each of the 1.5-kilobase inverted repeats of the kanamycin-resistance transposon Tn5 is transposable; we designate them IS50-L (left) and IS50-R (right). By DNA sequence analyses, IS50 is 1533 base pairs (bp) long and generates 9-bp direct repeats of target sequences. The ends of IS50 comprise a hyphenated 8-of-9-bp inverted repeat and are not used with equal efficiency; the outside ends are more active than the inside ends, suggesting that a strong transposase recognition site at the outside ends, suggesting that a strong transposase recognition site at the outside end extends beyond the 8 bp common to both ends.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=346254Documentos Relacionados
- Excision of transposon Tn5 is dependent on the inverted repeats but not on the transposase function of Tn5.
- Direct repeats flanking the Bacteroides transposon Tn4351 are insertion sequence elements.
- Specific binding of transposase to terminal inverted repeats of transposable element Tn3.
- Short interspersed repeats from Xenopus that contain multiple octamer motifs are related to known transposable elements.
- Nucleotide sequence of cloned unintegrated avian sarcoma virus DNA: viral DNA contains direct and inverted repeats similar to those in transposable elements.