InvestigaÃÃo do antagonismo entre Pseudomonas aeruginosa e bactÃrias do grupo coliforme

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

Pseudomonas aeruginosaâs interference against the coliform group in water analysis is known. Its enumeration has been questioned through the years because it is considered an opportunistic pathogen not inhabitant of intestinal tract of warm-blooded animals. Due to its metabolic versatility, it would have advantages under other organisms in water. In this work, 16 strains of P. aeruginosa isolated from water and wastewater samples, towards coliform bacteria were investigated. On solid medium all strains had antagonistic activity. The three most harmful strains from the qualitative test were chosen to be studied towards coliform bacteria (initial inoculum about 102CFU/mL) in 10% Műeller-Hinton broth. It was given to P. aeruginosa 72h of growth advantage. The test was carried out between 24h and 96h after the contact. Most-Probable-Number per 100mL of both populations was enumerated by using the multiple tube technique. The antagonistic phenomenon was observed when the initial inoculum was established at 102 CFU/mL. It also was evidenced when pyocyanin were present. E. aerogenes has been more sensitive than E. coli. This study reinforces the discussion about the inclusion of P. aeruginosa as an additional tool to drinking water analysis

ASSUNTO(S)

piocianina grupo coliforme antagonismo pyocyanin p. aeruginosa water quality pseudomonas aeruginosa antagonism qualidade de Ãgua quimica dos produtos naturais coliform group

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