Investigating antibody-catalyzed ozone generation by human neutrophils

AUTOR(ES)

Babior, Bernard M.

FONTE

The National Academy of Sciences

RESUMO

Recent studies have suggested that antibodies can catalyze the generation of previously unknown oxidants including dihydrogen trioxide (H2O3) and ozone (O3) from singlet oxygen (1O\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{*}}}\end{equation*}\end{document}) and water. Given that neutrophils have the potential both to produce 1O\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{*}}}\end{equation*}\end{document} and to bind antibodies, we considered that these cells could be a biological source of O3. We report here further analytical evidence that antibody-coated neutrophils, after activation, produce an oxidant with the chemical signature of O3. This process is independent of surface antibody concentration down to 50% of the resting concentration, suggesting that surface IgG is highly efficient at intercepting the neutrophil-generated 1O\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} \begin{equation*}{\mathrm{_{2}^{*}}}\end{equation*}\end{document}. Vinylbenzoic acid, an orthogonal probe for ozone detection, is oxidized by activated neutrophils to 4-carboxybenzaldehyde in a manner analogous to that obtained for its oxidation by ozone in solution. This discovery of the production of such a powerful oxidant in a biological context raises questions about not only the capacity of O3 to kill invading microorganisms but also its role in amplification of the inflammatory response by signaling and gene activation.

Documentos Relacionados

• Antibody-catalyzed reversal of chemoselectivity.
• Dissection of an antibody-catalyzed reaction.
• Toward antibody-catalyzed hydrolysis of organophosphorus poisons
• Probing the antibody-catalyzed water-oxidation pathway at atomic resolution
• Evidence for the production of trioxygen species during antibody-catalyzed chemical modification of antigens