Investigation of alveolar macrophage function using lucigenin-dependent chemiluminescence.
AUTOR(ES)
Williams, A J
RESUMO
The method using lucigenin-dependent phagocytic chemiluminescence is described for the assessment of alveolar macrophage metabolic activity in response to stimulation by opsonised particles or soluble agents. The requirement for superoxide anion (O2-) in the production of chemiluminescence is suggested by inhibition (95%) using superoxide dismutase. The results obtained are correlated with those obtained using another method of detecting O2- release (r = 0.61; p less than 0.05) and are also related by regression analysis to polymorphonuclear leucocyte contamination of the alveolar macrophage suspension. This shows that alveolar macrophages produce lucigenin-dependent chemiluminescence of the same order of magnitude as do polymorphonuclear leucocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=471831Documentos Relacionados
- Pattern of formylmethionyl-leucyl-phenylalanine-induced luminol- and lucigenin-dependent chemiluminescence in human neutrophils.
- Peritoneal macrophage activation indicated by enhanced chemiluminescence.
- Age-dependent variations in polymorphonuclear leukocyte chemiluminescence.
- Imaging of DNA sequences with chemiluminescence.
- Simple procedure for measuring neutrophil chemiluminescence.