Involvement of DNA polymerase alpha in host cell reactivation of UV-irradiated herpes simplex virus.
AUTOR(ES)
Nishiyama, Y
RESUMO
Aphidicolin is a potent inhibitor of both host cell DNA polymerase alpha and herpes simplex virus (HSV)-induced DNA polymerase but has no effect on DNA polymerases beta and gamma of host cells. By using an aphidicolin-resistant mutant (Aphr) of HSV, a possible involvement of DNA polymerase alpha in host cell reactivation of UV-damaged HSV was studied. Plaque formation by UV-irradiated Aphr was markedly inhibited by 1 microgram of aphidicolin per ml, which did not affect the plating efficiency of nonirradiated Aphr. Aphidicolin added before 12 h postinfection inhibited plaque formation by irradiated Aphr, which became aphidicolin insensitive after 36 h postinfection. The results strongly suggest that host cell DNA polymerase alpha is involved in the repair of UV-irradiated HSV DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255505Documentos Relacionados
- Quantitation of the viral DNA present in cells transformed by UV-irradiated herpes simplex virus.
- Quantitation of the Viral DNA Present in Cells Transformed by UV-Irradiated Herpes Simplex Virus
- Dihydrothymine from UV-irradiated DNA.
- DNA ligase activity in UV-irradiated monkey kidney cells.
- Reactivation of Photoinactivated Single-Stranded DNA Bacteriophage φX174 by UV-Irradiated Escherichia coli Cells