Iodometric Detection of Haemophilus influenzae Beta-Lactamase: Rapid Presumptive Test for Ampicillin Resistance
AUTOR(ES)
Catlin, B. Wesley
RESUMO
Strains of Haemophilus influenzae type b sporadically isolated from clinical specimens are ampicillin resistant due to production of a β-lactamase. This enzyme which inactivates ampicillin and penicillin G is not produced by ampicillin-susceptible strains. Various characteristics of β-lactamase production and ampicillin resistance of three H. influenzae type b isolates were investigated. A sensitive iodometric test was employed to detect β-lactamase; positive results were obtained in 5 min with 109 bacteria taken from cultures on a nutritionally adequate agar medium. This simple chemical test will enable the hospital laboratory to obtain presumptive evidence of ampicillin resistance on the same day that H. influenzae is isolated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=429122Documentos Relacionados
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