IRON: ITS INTRACELLULAR LOCALIZATION AND POSSIBLE ROLE IN CELL DIVISION*
AUTOR(ES)
Robbins, Elliott
RESUMO
When HeLa cells are fixed with ethanol, extracted with 0.1 N HCl, and incinerated at 540°C, all organic constituents and all ions studied are removed with the exception of iron. The gross outlines of cell structures are preserved and high concentrations of residual ash in interphase nucleoli and mitotic chromosomes suggest that there may be a shift in iron salts during the cell cycle. Experiments with cells isotopically labeled in proteins, nucleic acids, lipids, and polysaccharides indicate that the iron is bound to a polysaccharide. Addition of iron chelating agents to living cells causes a selective inhibition of DNA synthesis. These data suggest that iron may play a crucial role in the mitotic process.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=335812Documentos Relacionados
- Intramolecular Interactions Between the Src Homology 3 Guanylate Kinase Domains of Discs Large Regulate Its Function in Asymmetric Cell Division*
- Intracellular localization and effects on cell division of a plasmid blocked in deoxyribonucleic acid replication.
- Intracellular localization of phospholipid transfer activity in Rhodopseudomonas sphaeroides and a possible role in membrane biogenesis.
- Polar localization of the MinD protein of Bacillus subtilis and its role in selection of the mid-cell division site
- Characterization of Phloem Iron and Its Possible Role in the Regulation of Fe-Efficiency Reactions