IS1397 Is Active for Transposition into the Chromosome of Escherichia coli K-12 and Inserts Specifically into Palindromic Units of Bacterial Interspersed Mosaic Elements

AUTOR(ES)

Clément, Jean-Marie

FONTE

American Society for Microbiology

RESUMO

We demonstrate that IS1397, a putative mobile genetic element discovered in natural isolates of Escherichia coli, is active for transposition into the chromosome of E. coli K-12 and inserts specifically into palindromic units, also called repetitive extragenic palindromes, the basic element of bacterial interspersed mosaic elements (BIMEs), which are found in intergenic regions of enterobacteria closely related to E. coli and Salmonella. We could not detect transposition onto a plasmid carrying BIMEs. This unprecedented specificity of insertion into a well-characterized chromosomal intergenic repeated element and its evolutionary implications are discussed.

Documentos Relacionados

• Palindromic Unit-Independent Transposition of IS1397 in Yersinia pestis†
• Transposition of IS1397 in the Family Enterobacteriaceae and First Characterization of ISKpn1, a New Insertion Sequence Associated with Klebsiella pneumoniae Palindromic Units
• Transposases are responsible for the target specificity of IS1397 and ISKpn1 for two different types of palindromic units (PUs)
• Palindromic units are part of a new bacterial interspersed mosaic element (BIME).
• Escherichia coli K-12 envelope proteins specifically required for ferrienterobactin uptake.