Isolation and characterization of a bacteriophage specific for the lipopolysaccharide of rough derivatives of Pseudomonas aeruginosa strain PAO.
AUTOR(ES)
Jarrell, K F
RESUMO
A lipopolysaccharide (LPS)-defective (rough) mutant of Pseudomonas aeruginosa PAO was isolated by selection for resistance to the LPS-specific phage E79. The LPS of this mutant, AK-1012, lacked the O-antigenic side chain-specific amino sugar fucosamine as well as the core-specific sugars glucose and rhamnose. Using this strain, we isolated and characterized a phage, phi PLS27, which is specifically inactivated upon incubation with LPS extracted from rough mutants of P. aeruginosa PAO. phi PLS27 was found to be a Bradley type C phage and was very similar to coliphage T7 in a number of properties, including size, buoyant density, mass, and the number of structural proteins.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=171183Documentos Relacionados
- Identification of the cell wall receptor for bacteriophage E79 in Pseudomonas aeruginosa strain PAO.
- Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO.
- Isolation and genetic characterization of toxin-deficient mutants of Pseudomonas aeruginosa PAO.
- Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO.
- Genetic and physiological characterization of ciprofloxacin resistance in Pseudomonas aeruginosa PAO.
