Isolation and characterization of a low-abundance splice variant from the human cytomegalovirus major immediate-early gene region.
AUTOR(ES)
Kerry, J A
RESUMO
The major immediate-early (IE) gene region of human cytomegalovirus (HCMV) encodes several proteins as a result of differential RNA splicing events. By expression vector cloning of HCMV IE mRNA, we isolated and characterized a cDNA for a novel splice variant from the major IE gene region. The RNA product is a derivative of the IE55 mRNA and contains an additional splice from nucleotides 170,635 to 170,307 in the IE2 gene region (UL122), resulting in a 1.4-kb mRNA. The predicted open reading frame codes for a 164-amino-acid protein with a calculated molecular mass of 18 kDa (IE18). Mung bean nuclease analysis and PCR were used to characterize expression of IE18 mRNA in HCMV-infected cells. While the 1.4-kb mRNA was detected in infected human fibroblasts in the presence of a protein synthesis inhibitor, it was not detectable during a normal infection. However, the 1.4-kb mRNA was readily detected in infected human monocyte-derived macrophages at IE times. These results suggest that the novel IE18 mRNA exhibits cell type-specific expression indicating differential regulation of the major IE gene region in different permissive cell types.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=189107Documentos Relacionados
- Regulated expression of early and late RNAs and proteins from the human cytomegalovirus immediate-early gene region.
- The Human Cytomegalovirus Major Immediate-Early Distal Enhancer Region Is Required for Efficient Viral Replication and Immediate-Early Gene Expression
- Human cytomegalovirus immediate-early two protein region involved in negative regulation of the major immediate-early promoter.
- Autoregulation of the human cytomegalovirus major immediate-early gene.
- Effect of interstrain variation on diagnostic DNA amplification of the cytomegalovirus major immediate-early gene region.