Isolation and characterization of a serine proteinase with thrombin-like activity from the venom of the snake Bothrops asper
AUTOR(ES)
Pérez, A.V, Rucavado, A, Sanz, L, Calvete, J.J, Gutiérrez, J.M
FONTE
Brazilian Journal of Medical and Biological Research
DATA DE PUBLICAÇÃO
17/12/2007
RESUMO
A serine proteinase with thrombin-like activity was isolated from the venom of the Central American pit viper Bothrops asper. Isolation was performed by a combination of affinity chromatography on aminobenzamidine-Sepharose and ion-exchange chromatography on DEAE-Sepharose. The enzyme accounts for approximately 0.13% of the venom dry weight and has a molecular mass of 32 kDa as determined by SDS-PAGE, and of 27 kDa as determined by MALDI-TOF mass spectrometry. Its partial amino acid sequence shows high identity with snake venom serine proteinases and a complete identity with a cDNA clone previously sequenced from this species. The N-terminal sequence of the enzyme is VIGGDECNINEHRSLVVLFXSSGFL CAGTLVQDEWVLTAANCDSKNFQ. The enzyme induces clotting of plasma (minimum coagulant dose = 4.1 µg) and fibrinogen (minimum coagulant dose = 4.2 µg) in vitro, and promotes defibrin(ogen)ation in vivo (minimum defibrin(ogen)ating dose = 1.0 µg). In addition, when injected intravenously in mice at doses of 5 and 10 µg, it induces a series of behavioral changes, i.e., loss of the righting reflex, opisthotonus, and intermittent rotations over the long axis of the body, which closely resemble the `gyroxin-like' effect induced by other thrombin-like enzymes from snake venoms.
Documentos Relacionados
- Two related thrombin-like enzymes present in Bothrops atrox venom
- The complete nucleotide sequence of the gene for batroxobin, a thrombin-like snake venom enzyme.
- Caracterização bioquímica e funcional de uma nova Thrombin-like isolada da peçonha de Bothrops pauloensis
- Functional and structural characterization of phospholipases A² isolated from Bothrops asper snake venom in Panamá
- Purification and partial characterization of phospholipases A2 from Bothrops asper (barba amarilla) snake venom from Chiriguaná (Cesar, Colombia)