Isolation and characterization of a temperature-sensitive mutant of avian myeloblastosis virus.
AUTOR(ES)
Moscovici, M G
RESUMO
A temperature-sensitive (ts) mutant, GA 907/7, was isolated after mutagen treatment of avian myeloblastosis virus. When bone marrow cells or secondary yolk sac macrophages were infected with GA 907/7, the expression of transformation was greatly reduced at 41 degrees C. The results of temperature-shift experiments suggest that in GA 907/7 the putative v-myb gene product is functional only at 35.5 degrees C. Moreover, when ts-induced transformed cells were shifted to 41 degrees C, a partial morphological conversion to macrophage-like cells was obtained, while the majority of the cells underwent senescence and lysis. No leukemia was obtained when GA 907/7 was injected in 1-day-old chickens. Finally, a continuous cell line releasing genetically stable mutant virus was obtained after transformation of secondary yolk sac cells.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393609Documentos Relacionados
- Isolation and characterization of temperature-sensitive mutants of measles virus.
- Characterization of ts 16, a temperature-sensitive mutant of vaccinia virus.
- Isolation and preliminary characterization of temperature-sensitive mutants of measles virus.
- Isolation and preliminary characterization of temperature-sensitive mutants of encephalomyocarditis virus.
- Isolation and preliminary characterization of temperature-sensitive mutants of Newcastle disease virus.
