Isolation and Characterization of an Escherichia coli Bacteriophage Requiring Cell Wall Galactose

AUTOR(ES)

Watson, Gordon

RESUMO

A new coliphage, designated U3, has been selected for the ability to discriminate the presence of galactose in the cell wall of Escherichia coli. U3 attacks E. coli K-12 cells that are able to incorporate galactose into their cell walls, but mutants blocked in the synthesis of uridine diphosphogalactose, the precursor of cell wall galactose, are completely resistant to the phage. U3 is a small, tail-less, approximately spherical phage resembling φX174 in its physical properties. Its diameter by electron microscopy is 21 to 22 nm, and its particle weight is approximately 4 × 106 daltons. Like φX174, U3 appears to have a single-stranded deoxyribonucleic acid genome and has at least four cistrons.

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