Isolation and characterization of polyoma uncoating intermediates from the nuclei of infected mouse cells.
AUTOR(ES)
Winston, V D
RESUMO
A method was developed which enabled the efficient recovery of polyoma uncoating intermediates from the nuclei of infected cells at early times after infection (15 min to 12 h). Cells were infected with radiolabeled virus and lysed with the detergent Nonidet P-40. The nuclei were then collected and sonicated, and the products were analyzed on sucrose gradients. The uncoating intermediate sedimented at 190S and was a viral DNA-protein complex closely associated with a structure of host origin. The host material associated with the 190S uncoating intermediate was determined by polyacrylamide gel electrophoresis and visualized by electron microscopy. The amount of 190S uncoating intermediate found in the nucleus increased with time after infection. The viral DNA was predominantly for I. All of the viral proteins were present in the 190S uncoating intermediate in amounts similar to those found in viral DNA-protein complex cores.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=288650Documentos Relacionados
- Isolation and characterization of monopinocytotic vesicles containing polyomavirus from the cytoplasm of infected mouse kidney cells.
- DNA polymerases in polyoma virus-infected mouse kidney cells.
- DNA and Gene Therapy: Uncoating of Polyoma Pseudovirus in Mouse Embryo Cells*
- Isolation and characterization of cytoplasmic inclusions from influenza A virus-infected cells.
- Isolation of a Polyoma-Nucleoprotein Complex from Infected Mouse-Cell Cultures
