Isolation and Characterization of Tetracycline Resistance Proteins from Staphylococcus aureus and Escherichia coli
AUTOR(ES)
Wojdani, Aristo
RESUMO
Immunoglobulin (adsorbed resistance antiserum) reacting specifically with antigens from tetracycline (Tc)-resistant Staphylcoccus aureus or Escherichia coli was produced by adsorbing immunoglobulin against cell envelopes of resistant strains with envelope extracts from the respective isogenic susceptible strains. Adsorbed resistance antiserum against S. aureus reacted with envelope extracts from 32 Tc-resistant strains and failed to react with similar extracts from 76 Tc-susceptible strains of S. aureus. An antigen (Tc resistance antigen [TRA]) found only in Tc-resistant strains was produced by adsorbing envelope extracts from these strains with immunoglobulin against envelopes from isogenic Tc-susceptible strains. On immunodiffusion no cross-reactivity between TRAs from S. aureus and E. coli was observed. The TRAs behaved as proteins. The molecular weight of TRA from S. aureus was determined to be 32,000 and from E. coli to be 50,000. Data obtained by preliminary amino acid analysis of the TRAs are presented.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=429564Documentos Relacionados
- Isolation and Characterization of a Kanamycin Resistance Plasmid from Staphylococcus aureus
- Sequence of a class E tetracycline resistance gene from Escherichia coli and comparison of related tetracycline efflux proteins.
- Comparison of Tetracycline Action on Staphylococcus aureus and Escherichia coli by Microbial Kinetics
- Characterization of the tetracycline resistance gene of plasmid pT181 of Staphylococcus aureus.
- Biochemical and Genetic Basis of Tetracycline Resistance in Staphylococcus aureus
