Isolation and characterization of the centromere from chromosome V (CEN5) of Saccharomyces cerevisiae.
AUTOR(ES)
Maine, G T
RESUMO
We have cloned a functional centromeric DNA sequence from Saccharomyces cerevisiae. Using the 2 mu chromosome-loss mapping technique and meiotic tetrad analysis, we have identified this DNA sequence as the centromere of chromosome V (CEN5). The CEN5 sequence has been localized on an 1,100-base-pair BamHI-BglII restriction fragment. Plasmids containing CEN5 and an autonomously replicating sequence are mitotically stable in S. cerevisiae and segregate in a Mendelian fashion during meiosis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=368661Documentos Relacionados
- Identification and characterization of the centromere from chromosome XIV in Saccharomyces cerevisiae.
- Isolation and subcloning analysis of functional centromere DNA (CEN11) from Saccharomyces cerevisiae chromosome XI.
- Mutational analysis of centromere DNA from chromosome VI of Saccharomyces cerevisiae.
- Isolation and characterization of a dinucleoside triphosphatase from Saccharomyces cerevisiae.
- Alterations in the adenine-plus-thymine-rich region of CEN3 affect centromere function in Saccharomyces cerevisiae.