Isolation and characterization of two fractions from HeLa cells required for mRNA splicing in vitro.
AUTOR(ES)
Furneaux, H M
RESUMO
A nuclear extract from HeLa cells has been separated by DEAE-cellulose chromatography into two fractions, both of which are required for mRNA splicing in vitro. Both fractions are heat labile and sensitive to N-ethylmaleimide. The activity of one of the fractions was abolished by preincubation with micrococcal nuclease, while the other fraction was unaffected by this treatment. This abolition indicates an essential nucleic acid component. Fractions I and II are required for the in vitro splicing of human beta-globin and adenovirus transcripts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=390411Documentos Relacionados
- Isolation and characterization of an RNA ligase from HeLa cells.
- Adenovirus proteins associated with mRNA and hnRNA in infected HeLa cells.
- Poly(A) polymerase purified from HeLa cell nuclear extract is required for both cleavage and polyadenylation of pre-mRNA in vitro.
- In vitro deadenylation of mammalian mRNA by a HeLa cell 3' exonuclease.
- Comparison of nucleotide sequences in HeLa cell mRNA and hnRNA.