Isolation and expression of a rat brain cDNA encoding glutamate carboxypeptidase II
AUTOR(ES)
Luthi-Carter, Ruth
FONTE
The National Academy of Sciences
RESUMO
N-acetylated alpha-linked acidic dipeptidase (NAALADase) hydrolyzes acidic peptides, such as the abundant neuropeptide N-acetyl-α-l-aspartyl-l-glutamate (NAAG), thereby generating glutamate. Previous cDNA cloning efforts have identified a candidate rat brain NAALADase partial cDNA, and Northern analyses have identified a family of related RNA species that are found only in brain and other NAALADase-expressing cells. In this report, we describe the cloning of a set of rat brain cDNAs that describe a full-length NAALADase mRNA. Transient transfection of a full-length cDNA into the PC3 cell line confers NAAG-hydrolyzing activity that is sensitive to the NAALADase inhibitors quisqualic acid and 2-(phosphonomethyl)glutaric acid. Northern hybridization detects the expression of three similar brain RNAs approximately 3,900, 3,000, and 2,800 nucleotides in length. In situ hybridization histochemistry shows that NAALADase-related mRNAs have an uneven regional distribution in rat brain and are expressed predominantly by astrocytes as demonstrated by their colocalization with the astrocyte-specific marker glial fibrillary acidic protein.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=19722Documentos Relacionados
- Isolation and developmental expression of a rat cDNA encoding a cysteine-rich zinc finger protein.
- Isolation and expression of a cDNA encoding Renilla reniformis luciferase.
- Isolation of cDNA and genomic DNA clones encoding type II collagen.
- Isolation and expression of cDNA clones encoding mammalian poly(A) polymerase.
- Cloning and characterization of a cDNA encoding the rat brain glucose-transporter protein.
