ISOLATION AND INCIDENCE OF PLEUROPNEUMONIA-LIKE ORGANISMS FROM THE HUMAN ORAL CAVITY1

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Shklair, I. L. (U. S. Naval Training Center, Great Lakes, Ill.), M. A. Mazzarella, R. R. Gutekunst, and E. M. Kiggins. Isolation and incidence of pleuropneumonia-like organisms from the human oral cavity. J. Bacteriol. 83:785–788. 1962.—Pleuropneumonia-like organisms (PPLO) were isolated from the oral cavity of 87.2% (184 of 211) of young male naval recruits, by use of an enriched medium under anaerobic conditions. Aerobically, the organisms were isolated from 75% (78 of 104) of the subjects. However, aerobic cultivation was not the method of choice for the isolation of these organisms, as only a few colonies of PPLO were found. These colonies were atypical, or incomplete, and could not be further subcultured either aerobically or anaerobically. With anaerobic procedures, typical PPLO colonies developed and they could be subcultured anaerobically.

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